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Drilling groups tested in vitro.
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Establishment of a <t>microfluidic</t> system using an organ-on-chip system and feline mammary tumor (FMT) organoids. Overview of organoids-on-a-chip experiments ( A ). Representative H&E-stained image (Left panel) and expression of a mammary tumor marker HER2 (Right panel) in FMT organoids are shown ( B ). Scale bar: 50 μm. After generating organoids from a cat with mammary tumor disease, the organoids were mounted on a microfluidic device, and drug perfusion was performed. After perfusion, the organoids were collected for genetic analysis and cell survival measurements. The device was created by CAD design and acrylic cutting. The width and height of the flow channel were 0.4 and 0.5 mm, respectively, and the diameter of the chamber was set at 5 mm ( C ). Liquid flows in from the left side of the device and flows out in three separate directions ( D ). The device was connected to a syringe pump and perfused in a CO 2 incubator at 37 ℃ for 48 h ( E ).
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Image Search Results


Drilling groups tested in vitro.

Journal: Bioengineering

Article Title: Comparison of One-Drill Protocol to Sequential Drilling In Vitro and In Vivo

doi: 10.3390/bioengineering12010051

Figure Lengend Snippet: Drilling groups tested in vitro.

Article Snippet: Thermal video recordings taken during the implant bed preparation procedure for the 4.0 mm diameter new-generation drill bit used in a one-drill protocol and Straumann BLT drill bits used in sequential drilling were analyzed and maximum temperatures at osteotomy depths of 0, 2, 4, 6, 8, and 10 mm were recorded and organized into the bar graph seen in .

Techniques:

Animal study groups.

Journal: Bioengineering

Article Title: Comparison of One-Drill Protocol to Sequential Drilling In Vitro and In Vivo

doi: 10.3390/bioengineering12010051

Figure Lengend Snippet: Animal study groups.

Article Snippet: Thermal video recordings taken during the implant bed preparation procedure for the 4.0 mm diameter new-generation drill bit used in a one-drill protocol and Straumann BLT drill bits used in sequential drilling were analyzed and maximum temperatures at osteotomy depths of 0, 2, 4, 6, 8, and 10 mm were recorded and organized into the bar graph seen in .

Techniques: Control, Modification

Establishment of a microfluidic system using an organ-on-chip system and feline mammary tumor (FMT) organoids. Overview of organoids-on-a-chip experiments ( A ). Representative H&E-stained image (Left panel) and expression of a mammary tumor marker HER2 (Right panel) in FMT organoids are shown ( B ). Scale bar: 50 μm. After generating organoids from a cat with mammary tumor disease, the organoids were mounted on a microfluidic device, and drug perfusion was performed. After perfusion, the organoids were collected for genetic analysis and cell survival measurements. The device was created by CAD design and acrylic cutting. The width and height of the flow channel were 0.4 and 0.5 mm, respectively, and the diameter of the chamber was set at 5 mm ( C ). Liquid flows in from the left side of the device and flows out in three separate directions ( D ). The device was connected to a syringe pump and perfused in a CO 2 incubator at 37 ℃ for 48 h ( E ).

Journal: Scientific Reports

Article Title: A microfluidics platform for simultaneous evaluation of sensitivity and side effects of anti-cancer drugs using a three-dimensional culture method

doi: 10.1038/s41598-024-84297-0

Figure Lengend Snippet: Establishment of a microfluidic system using an organ-on-chip system and feline mammary tumor (FMT) organoids. Overview of organoids-on-a-chip experiments ( A ). Representative H&E-stained image (Left panel) and expression of a mammary tumor marker HER2 (Right panel) in FMT organoids are shown ( B ). Scale bar: 50 μm. After generating organoids from a cat with mammary tumor disease, the organoids were mounted on a microfluidic device, and drug perfusion was performed. After perfusion, the organoids were collected for genetic analysis and cell survival measurements. The device was created by CAD design and acrylic cutting. The width and height of the flow channel were 0.4 and 0.5 mm, respectively, and the diameter of the chamber was set at 5 mm ( C ). Liquid flows in from the left side of the device and flows out in three separate directions ( D ). The device was connected to a syringe pump and perfused in a CO 2 incubator at 37 ℃ for 48 h ( E ).

Article Snippet: After milling the microfluidic features, a 1 mm diameter drill bit (MHR230, NS Tool Co., Ltd., Japan) was used to segment the acrylic sheet into individual sections, each measuring 3 × 4 cm and containing a complete microfluidic channel network.

Techniques: Staining, Expressing, Marker